A simple assay method for erythropoietin in vitro.

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In Vitro Assay for Erythropoietin: Erythroid Colony Formation in Methyl Cellulose Used for the Measurement of Erythropoietin in Plasma

Erythroid colony formation in methyl cellulose has been used for the measurement of erythropoietin in plasma. Livers from newborn mice less than 24 hr old were found to provide convenient target cells. Newborn mouse liver contains a substantial number of erythroid colony-forming cells (CFU-e) that have a high sensitivity to erythropoietin. the dose-response curve for erythropoietin reaching a p...

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1983

ISSN: 0002-1369,1881-1280

DOI: 10.1271/bbb1961.47.1311